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plasmid that drives dcas9-dnmt3a-t2a-egfp expression with cmv promoter/enhancer  (Addgene inc)


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    Addgene inc plasmid that drives dcas9-dnmt3a-t2a-egfp expression with cmv promoter/enhancer
    Plasmid That Drives Dcas9 Dnmt3a T2a Egfp Expression With Cmv Promoter/Enhancer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid that drives dcas9-dnmt3a-t2a-egfp expression with cmv promoter/enhancer/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    plasmid that drives dcas9-dnmt3a-t2a-egfp expression with cmv promoter/enhancer - by Bioz Stars, 2026-03
    90/100 stars

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    (A) AAV-CIRTS-gRNAluc significantly down-regulated luciferase expression in HEK293T cells after transient co-transfection with AAV-SFFV- luciferase -T2A- eGFP plasmid. Luciferase expression normalised to protein concentration within lysate. Unpaired, two-tailed t-test.

    Journal: bioRxiv

    Article Title: AAV delivery of RNA editing machinery rescues SUDEP and seizure phenotype in a mouse model of Dravet Syndrome

    doi: 10.1101/2025.07.18.664895

    Figure Lengend Snippet: (A) AAV-CIRTS-gRNAluc significantly down-regulated luciferase expression in HEK293T cells after transient co-transfection with AAV-SFFV- luciferase -T2A- eGFP plasmid. Luciferase expression normalised to protein concentration within lysate. Unpaired, two-tailed t-test.

    Article Snippet: At 6DIV, cells were transfected with 0.8μg AAV-SFFV- luciferase -T2A- eGFP or AAV-CIRTS-gRNA1-21 using Lipofectamine 2000 (Thermo Fisher Scientific).

    Techniques: Luciferase, Expressing, Cotransfection, Plasmid Preparation, Protein Concentration, Two Tailed Test